CHAPTER 1 - SYSTEM OPERATIONZEISS Left Tool Area and Hardware Control Tools LSM 880392 000000-2071-464 10/2014 V_015.4.22.2 Interpolate Brightness and ContrastThis function permits a continuous contrast andbrightness adjustment in a Z-Stack or Z-Stacks overtime by interpolation between the starting and endvalues of the respective image series. This permits apost-acquisition compensation of signal loss in, forexample, thick tissue imaging where excitation anddetection efficiency decrease significantly.Interpolation can be defined for the entire image oronly for individual channels. In addition, a set ofcoordinates for which intensities are kept fixed duringthe interpolation calculation can be defined (Fig. 548).The Select button selects the active image in theImage Display as the Input Image to be processed.In the Interpolation panel (Fig. 549), the parametersfor the interpolation procedure are set.• In the Coordinate pull down, select the dimensionfor the interpolation. Depending on the imagedata type, x, y, z, and time are the possibleoptions.• Use the List size spin box to set the number offixed data points. The table in the Interpolationpanel will be extended to the selected number ofrows.• Highlight a row of your choice by clicking on it.Then use the Brightness, Contrast and Z (or t)slider to set the values for this fixed data point. Thenumbers in the table will be updated according tothe slider position. Then highlight the next row, setthe parameters and keep setting the fixed datapoints for all rows.• Use the available All, Ch1-T1 and Ch2-T2 channelbuttons to select the channel for interpolation orclick on the All button if the entire image is to beinterpolated.• Having set the parameters, click on the Applybutton. Interpolation will be performed in a newImage Display window.• The newly created image (series) can be storedusing the Save As function.If you de-activate the Extrapolate check box,only the slices lying between the first and lastfixed data point (table rows) will be taken intoaccount for interpolation. Otherwise, brightnessand contrast will also be changed for the otherslices.Fig. 548 Processing – Adjust withInterpolate brightness andcontrastFig. 549 Processing – Adjust withInterpolate brightness andcontrast tool with loadedZ-Stack
