CHAPTER 1 - SYSTEM OPERATIONZEISS Left Tool Area and Hardware Control Tools LSM 880186 000000-2071-464 10/2014 V_015.3.2.5 Pinhole AdjustmentThe Adjust Pinhole panel allows you to set the pinhole diameter and to automatically align the pinholeposition in x and y.• Click on the Adjust Pinhole tab to open the Pinhole configuration panel (Fig. 269). Click on Closeto leave the panel.Setting the Pinhole diameterActivate the channel you want to use andthat is appropriate for your settings bypressing the respective Ch tab. In onechannel settings only the active used channelwill be available. In two or more channelsettings both channels will be available. Youcan adjust the pinhole to either channel. Thevalues for the pinhole alignment done withthe last channel will be stored and used witha method.You can set the pinhole diameter of the LSM usingthe Diameter slider, scroll arrows or the input box(Fig. 269). The display of the input box gives thediameter in μm. The Pinhole diameter in Airy unitsis calculated automatically and displayed beside theinput box. The 1 AU button allows you to set thepinhole size to 1 Airy unit by just clicking on it. Setthe desired pinhole diameter using the slider, thescroll arrows or type into the input box.This will alter the pinhole diameter automatically also for the method in the Pinhole panel. If youneed another pinhole size for doing the pinhole alignment be sure to reset it before saving themethod.• It is recommended to set the pinhole diameter to a size corresponding to the used excitationwavelength that corresponds to 1 AIRY unit:− 458 nm: 33 μm− 488 nm: 34 μm− 514 nm: 39 μm− 561 nm: 40 μm− 633 nm: 45 μmPinhole sizes are given in μm and AIRY units.Please note that there is only one pinhole for all detector channels of the various modules. NDDdoes not have a pinhole. You can adjust the pinhole to either channel. The values for the pinholealignment done with the last channel will be stored along with the beam path or a method andused. A method is always saved together with the system configuration, acquisition and processingparameters. A beam path is saved only with the pinhole settings.You can adjust the beam path to the pinhole of the LSM manually or automatically. Manual adjustment isnot recommended, since the relevant procedure is very complex.Fig. 269 Adjust Pinhole panel
