CHAPTER 1 - SYSTEM OPERATIONZEISS Left Tool Area and Hardware Control Tools LSM 880138 000000-2071-464 10/2014 V_015.2.14 Acquisition Parameter – RegionsThe Regions tool tab is only available, if theRegions checkbox is ticked in theMultidimensional acquisition field. If the tool bar isexpanded, the Regions tool becomes available(Fig. 185).The Regions tool allows the user to define Regionsof Interest (ROI) which are used for display for WF,SIM and Laser WF imaging modes as well asimage acquisition, sample manipulation (bleaching)and image analysis for LSM imaging mode.The control of the laser in LSM imaging mode viaAOTF technology enables the restriction of theillumination to the defined region. The sample isonly illuminated when data are acquired. Inwidefield techniques (WF, SIM, LSM WF), thewhole sample is always illuminated, even if onlythe region is displayed.The definition of the regions is done using anactual image of the sample and drawing theregions into this image (Fig. 186, Fig. 187). Thismakes it extremely easy to set the regions in theright position and dimension.Fig. 185 Regions toolFig. 186 Image with two defined regions,the square one is selectedFig. 187 Regions tool
