CHAPTER 1 - SYSTEM OPERATIONLSM 880 Left Tool Area and Hardware Control Tools ZEISS10/2014 V_01 000000-2071-464 1795.3.2.1 Light Path for 2 Channel Module(BiG.2 on DC out port)BiG.2 has two GaAsP detectors that can be usedsimultaneously to acquire two autocorrelationfunctions (AC) per each channel. There are alsotwo cross-correlation (CC) channels available,where the channels are correlated to each other.Selection of dichroics and filtersFrom top to bottom you have the followingdichroics and filters available.Main dichroic beam splitter (MBS):The MBS reflects the specified laser lines andallows the resulting fluorescence spectrum to passthrough.• Click on the MBS icon to open a list of dichroics(Fig. 261).• Select the appropriate one by a mouse click.• Close the list by clicking the icon again.• You have two major beam splitters, one for the Visible light and one of the Invisible light (ifapplicable). A plate should be placed in the dichroic that will not be used. If the system is notequipped with a violet laser line, the Invisible light major beam splitter is not available.Secondary dichroic beam splitter (SBS):The SBS splits the fluorescence spectrum onto the various detection channels. The long pass (LP) filter letswavelengths pass above the specified border wavelength and reflects wavelengths below. The short pass(SP) filter lets wavelengths pass below the specified border wavelength and reflects wavelengths above.The SBS is positioned in a 45° angle to the incident beam.The icon is inactive, as the SBS is part of the filter cube together with the emission filters, which has to bereplaced manually.Emission filter 1 and 2 (EF1 and EF2):The EF is used to narrow the fluorescence spectrum. The band pass (BP) filter allows the range within thespecified border wavelengths to pass through. The long pass (LP) filter allows the range above thespecified border wavelength to pass through. The short pass (KP) filter allows the range below thespecified border wavelength to pass through. The EFs are positioned in a 90° angle to the incident beam.The secondary beam splitter within the scan head, which deflects the signal either to the 2channel module (BiG.2) or to the 3/32 channel is set automatically to mirror/plate as needed.Fig. 261 Light Path tool for 2 ChannelModule
