CHAPTER 1 - SYSTEM OPERATIONLSM 880 Left Tool Area and Hardware Control Tools ZEISS10/2014 V_01 000000-2071-464 4115.5.5.3 System Options – HardwarePress the Hardware tab to open the Hardwarepanel (Fig. 580).Hardware lists parameters that, when theirrespective check box is ticked, will set severalhardware defaults.By activation of the Lasers off on exit check boxthe lasers are automatically switched off when theZEN software is closed. The lasers are allowed tocool for five minutes before they are switched off.By ticking the use argon laser line selection filter wheel (only applicable in LSM imaging mode) theline selection filter in the LSM head is used for additional excitation laser line suppression.If the Keep raw data for photon count detectors (only applicable for the LSM imaging mode) isticked, the actual photon counts will be assigned to each pixel and displayed in histograms. If no tickedphoton counts will be converted dependent on the color depth into grey values.The Z-Stack Waitstate function delays the image acquisition during time series or Z-Stacks in casevibrations of the stage occur and are registered at the beginning of individual images. The default is off.The delay is set to 350 ms. This can occur for specific image acquisition settings and is dependent on thespeed, the stage and the objective in use.Online scanner calibration (OSC) (only applicable for LSM imaging mode) assures the calibration ofthe scanners for uni- and bidirectional scanning is on as default setting. The default is on and it isrecommended to keep the calibration on at all times. If that function is not checked it might be requiredto calibrate the scanners in x and y manually when bidirectional scanning is used for image acquisition.Nonlinear bidirectional distortions especially at high acquisition speeds (10-15) can only be removed byOSC.The function will not be activated automatically upon starting the system if it has beendeselected before shutting down the system. When starting the software, a message will remindyou of activating the online scanner calibration.Fig. 580 System Options – Hardware panel
