CHAPTER 1 - SYSTEM OPERATIONZEISS Contents LSM 8802 000000-2071-464 10/2014 V_014.5.3 Help – About ..................................................................................................................374.6 Main Toolbar ................................................................................................................384.7 Status Bar......................................................................................................................404.8 Left Tool Area ...............................................................................................................414.8.1 Tool Groups and Tools.....................................................................................................434.8.2 Context Menu of the Left Tool Area.................................................................................444.9 Center Screen Area .......................................................................................................454.9.1 Overview of the Center Screen Area ................................................................................454.9.2 Context Menu of the Center Screen Area.........................................................................474.10 Right Tool Area.............................................................................................................485 LEFT TOOL AREA AND HARDWARE CONTROL TOOLS .....................................505.1 Locate Tab.....................................................................................................................515.1.1 Microscope Control Tool for Axio Observer.Z1 .................................................................535.1.2 Microscope Control Tool for Axio Imager.Z2 ....................................................................605.1.3 Controls for Axio Examiner ..............................................................................................635.1.4 Incubation Tool ...............................................................................................................645.2 Acquisition Tab .............................................................................................................655.2.1 Configuration Functions ..................................................................................................665.2.2 Smart Setup ....................................................................................................................685.2.3 Action Buttons ................................................................................................................735.2.4 Multidimensional Acquisition Settings ..............................................................................755.2.5 Setup Manager – Laser ....................................................................................................775.2.6 Setup Manager – Experiment Designer .............................................................................805.2.7 Setup Manager – Imaging Setup ......................................................................................845.2.7.1 Imaging Mode and Track Definition .................................................................................845.2.7.2 Filter Cube Selection ........................................................................................................865.2.7.3 Objective Selection ..........................................................................................................865.2.7.4 Stage Control..................................................................................................................875.2.7.5 Focus Control..................................................................................................................885.2.7.6 HAL Control ....................................................................................................................885.2.7.7 X-Cite Control .................................................................................................................895.2.7.8 Aperture Control .............................................................................................................895.2.7.9 Ratio Imaging ..................................................................................................................905.2.7.10 Laser Control ..................................................................................................................905.2.7.11 Control Elements of the Imaging Setup Tool ....................................................................915.2.7.12 Setup Imaging with Airyscan Detector..............................................................................995.2.8 Acquisition Parameter – Acquisition Mode .....................................................................1015.2.8.1 Objective Selection ........................................................................................................1015.2.8.2 Scan Mode....................................................................................................................1025.2.8.3 Format Selection ...........................................................................................................1025.2.8.4 Averaging and Color Depth Selection.............................................................................1035.2.8.5 HDR(High Dynamic Range)-Imaging ...............................................................................1045.2.8.6 Scan Area Definition ......................................................................................................1055.2.8.7 Acquisition Mode Functions for LSM ..............................................................................1065.2.8.8 Acquisition Mode Settings for Airyscan ..........................................................................1135.2.9 Acquisition Parameter – Channels ..................................................................................115
