CHAPTER 1 - SYSTEM OPERATIONLSM 880 Hardware Aspects ZEISS10/2014 V_01 000000-2071-464 132.4 Optical Diagram of the LSM 880 (Schematic)AS Airyscan detector MBS Main Beam SplitterDBS Dichroic Beam Splitter NDD Non-Descanned DetectorEF Emission Filter PMT PhotomultiplierHAL Halogen Lamp T-PMT Transmission-PhotomultiplierHBO Mercury Vapor Short-Arc Lamp ZO Zoom OpticsIBF Infrared Blocking Filter GaAsP Gallium Arsenide PhosphideFig. 4 Optical path on an inverted microscope, schematic (2 PMT / 32 array configuration)The diagram above is a schematic representation of the LSM 880 system with attached Airyscan andBiG.2 module.Laser light is focused onto the specimen through an objective in a diffraction-limited mode. Light emittedat the focal plane and at planes below and above it is directed via an XY scanner onto a main dichroicbeam splitter (MDBS), which separates the emissions from the excitation light. In the LSM 880 thefluorescence is either spectrally separated by passing through a grating onto the Quasar detector (3 PMTsor 2 PMTs plus 1 central GaAsP detector or 2 PMTs plus 32 channel GaAsP array) or directed viasecondary beam splitters into Airyscan or BiG.2 module. In Airyscan light is filtered by emission filters(EF5) and projected onto the Airyscan detector with its 32 sub-airy detector elements. In the BiG.2module the light passes through a Push & Click filter cube with a dichroic beam splitter (DBS 6) and twoemission filters (EF6 and EF7) onto 2 GaAsP (GaAsP1 and GaAsP2) detectors. The pinhole (VP) blocks allout of focus light, whereas emission filters (EF) are used to select a specific spectrum of the emitted lightand to suppress any remaining excitation light. In NLO applications, light can be either directed to theQuasar detector or BiG.2 module (descanned detection) or to NDDs (equipped with either multialkali orGaAsP PMT) in transmission or reflection position (non-descanned detection).
