CHAPTER 1 - SYSTEM OPERATIONLSM 880 Left Tool Area and Hardware Control Tools ZEISS10/2014 V_01 000000-2071-464 3475.4.17.2 Structured Illumination – StructuredIlluminationThe Structured Illumination function allows automaticor manual processing of a set of structured imagesobtained by phase shift and rotation of the grid.Select the Structured Illumination tab to select theStructured Illumination Processing tool (Fig. 479). Activate the image you want to process in theimage container and press the Select button to load theimage. Then expand the Method Parameters toolwindow to have access to setting parameters (Fig. 480).You have to use the subdirectory StructuredIllumination tab when selecting the image tohave aces to the Structured Illumination tools.You will have no tools available is the directoryStructured Illumination tab is selected.If a region of interest (ROI) is defined in the image, thenthis ROI will be exclusively taken for image processingand the rest of the image will not be processed.You can instead of processing only one selected imagealso load more images to be processed sequentially. Tothis end press the Run Batch button (Fig. 480). This willopen Windows Explorer from which you can select allimages you want to process. All selected files will bedisplayed in the Batch display window, which willappear when the files are opened by Windows explorer.The Batch display window also shows the current stateof batch processing.Fig. 479 Processing – Structuredillumination tool selectedFig. 480 Structured Illumination –Structured Illumination
