CHAPTER 1 - SYSTEM OPERATIONLSM 880 Left Tool Area and Hardware Control Tools ZEISS10/2014 V_01 000000-2071-464 1695.2.23 Image OptimizationThis section describes an example how to acquirean image, using an excitation wavelength of561 nm and a fluorescence emission range above570 nm. Use the MBS 488/561 as the maindichroic beam splitter. We presume that nopredefined configuration is available for thisimaging task.The description applies to the use of anymicroscope.Data acquisitions is impaired whenkeeping the computer busy with othertasks then acquisition like processing dataor using other software in the mean time.This might slow down image acquisitionand can even interfere with the timing ofthe acquisition.5.2.23.1 Requirements• Position the specimen under the objective andfocus onto the desired imaging plane.• Activate Show all Tools and open theImaging Setup tool. Chose Channel mode forthe image acquisition (Fig. 249).• Switch on the Laser 561 nm.• Click on the laser icon, select the 561 line, andset the transmission slider to about 3 % (thislaser has an output power of 10 mW, otherlasers have up to 100 mW depending on thetype of laser and the imaging device used; becareful when setting the power for imagingwith other lasers for imaging other dyes). Makesure other lasers are not checked (Fig. 250).Optimize the laser intensity subsequentlyvia the slider when imaging.• Activate channel 1 (Ch1) via the check box(Fig. 249).Fig. 249 Imaging Setup – imaging modeselection and track activationFig. 250 Laser line and laser power selection
