CHAPTER 1 - SYSTEM OPERATIONZEISS Left Tool Area and Hardware Control Tools LSM 880412 000000-2071-464 10/2014 V_015.5.5.4 System Options – Image DisplayPress the Image display tab to open the Imagedisplay panel (Fig. 581).If the Apply brightness and contrast changes todiagrams and tables is ticked any changes inbrightness and contrast of the image will alsoaffect the image data shown in diagrams or tables.If not ticked any changes in the Image Display willnot affect the original image data. The defaultsetting is off.The Temporary files directory can be chosenmanually by pressing the … button via Windowsexplorer. For large datasets it is advisable to use adrive other than the C-Drive of your computer.When ticking the Interpolate checkbox Interpolates the signal between pixels and thus smoothens theimage. This interpolation is not saved with the original image data and can be active also duringacquisition.Ticking the Invert x check box will invert the x-coordinate in the display. The original data set is notchanged.Ticking the Invert y check box will invert the y-coordinate in the display. The original data set is notchanged.Ticking the Invert z check box will invert the z-coordinate in the display. The original data set is notchanged.Ticking the Invert time (line scan only) check box (only available in LSM imaging mode in Line Scan)will reverse the time points. The original data set is not changed.Ticking the Exchange x and y check box will exchange x for y coordinates and vice versa. The originaldata set is not changed.Ignore file sets provides the possibility to open all files, including the master file, as a separate file.Master files are generated when images are saved in individual files along time point, stage position, tile,Z stack position and/or channel. This is defined within the Autosave Tool. Per default the option is notactive and such subfiles are opened as one file if the master file (the first file set of all) is opened. Only thesubsequent files can be opened individually unless this function is active.Note: When a file set is opened via the masterfile and modified (i.e. cut region, subset etc.) and thensaved again, the new data set will be saved only in one file and not in file subsets like the original data.Airyscan Processing Baseline Shift sets the values of the histogram in the Display function tab of theAiryscan View tab to a new grey level baseline of 10.000. Negative values will be displayed as beingsmaller than 10.000 and can be displayed if wanted. If this function is inactive, negative values will be cutfrom the histogram and the baseline is set to 0.For changing this setting you have to (store and) reload an image.Due to the nature of the Airyscan detection and the signal reassignment to the respective airy orders, thedisplay curves (histogram) of the processing preview are complex and have a wide dynamic range,though the majority of the signal will be at a small number of grey values. To get a balanced view, thedisplay settings are adjusted automatically. Also, a baseline shift will be set. By default, this AiryscanProcessing Baseline Shift checkbox is activated.Optionally, this baseline shift can be deactivated. This allows the display of negative intensity results ofthe processing. Such results will be displayed in the range from 0 to 10,000 in the grey range histogram.Fig. 581 System Options – Image display
