CHAPTER 1 - SYSTEM OPERATIONLSM 880 Left Tool Area and Hardware Control Tools ZEISS10/2014 V_01 000000-2071-464 3935.4.22.3 Channel ShiftChannel shift is used to produce a congruentimage with relation to the pixels of the variouschannels (Fig. 550).This pixel correction function is particularlyimportant in UV applications.This is a manipulation of your data that hasto be considered very carefully when co-localization analysis is part of yourexperiment.The Select button selects the active image in theImage Display as the Input Image.The image has to be a multi-channel image.• Select the channels you want to shift by tickingthe Ch1-T1 or Ch2-T2 check box:• Use the X and Y sliders or spin boxes to definethe shift in pixels to select the pixel shift in thehorizontal and vertical direction. Clicking theZero next to the spin-boxes resets the shift tothe original position.A preview is automatically generated at thebottom of the Channel Shift tool in theProcessing tab of the Left Tool Area At the sametime, a large preview is shown in the Previewview tab in the Image display in the Center ScreenArea.• Click on the Apply button to generate a newimage with the set pixel shift.Fig. 550 Processing – Adjust with Channelshift
