CHAPTER 1 - SYSTEM OPERATIONLSM 880 Left Tool Area and Hardware Control Tools ZEISS10/2014 V_01 000000-2071-464 735.2.3 Action ButtonsAction buttons provide control for how the image is acquired (Fig. 76).Fig. 76 Action buttonsThey have the following functions, regardless of the imaging mode:AF Find Focus triggers a frame z-Scan. The system selects the z-position associated with the frame ofhighest intensity or contrast. AF Find Focus can be disrupted by pressing the Stop button, to which theSnap button changes upon activation (Fig. 77).Fig. 77 Action buttons – AF Find Focus button activeSet Exposure optimizes the gain and offset settings (PMT only, for cameras only the exposure time willbe adjusted) of all active channels and tracks dependent on the given laser power. Set Exposure can bedisrupted by pressing the Stop button, to which the Set Exposure button changes upon activation(Fig. 78).Fig. 78 Action buttons – Set exposure button activeLive uses the maximum speed for a given image frame (and zoom setting) for all active channels. It wilset the resolution to 248 x 248 for the Andor SIM camera, 256 x 256 for the Andor PALM camera and512 by 512 for single point detectors (PMT, GaAsP). To disrupt Live acquisition, the Stop button has tobe pressed into which the Live button changes after its activation (Fig. 79).Fig. 79 Action buttons – Live button active
