Zeiss LSM 510 Operating Manual

Also see for LSM 510 Inverted: Quick guide Operating manual User guide User manual Brief Operating Manual

OPERATIONImage Acquisition (Acquire) LSM 5104-56 B 40-51 e 07/98 On the Zoom, Rotation & Offset panel, use the slider to set the desired zoom factor between 1and 8. This adjustment is infinite. The scanning frame thus defined is shown by a red outline.The scanning frame can be rotated and translated at liberty.Recommended setting Zoom 1to start with: Rotation 0Please bear in mind that a reference scale which might be included in the image during the scanningprocedure will only be activated after the end of the scanning procedure if you change the zoom factor Click on in the upper toolbar. This opens the new dialog boxes ChannelSettings and Excitation of Track, showingthe previously set parameters, which can,however, be edited. Optimize the image quality by varying thepinhole aperture: A small pinhole diameter will increase thedepth of focus, but reduce the light intensityreceived by the PMT. When you vary the pinhole diameter, anOptical Slice value is displayed. For optimumdepth resolution, Airy values should besmall, but not below 1.0 to keep theintensity loss within a reasonable limit.+ The parameters Detector Gain Ampl. Offset Ampl. Gainare described in sections 4.7.1 in thecontext of image optimization. In the Excitation of Track dialog box you canselect other lasers and vary laser intensities.Fig. 4-56