Zeiss LSM 510 Operating Manual

Also see for LSM 510 Inverted: Quick guide Operating manual User guide User manual Brief Operating Manual

OPERATIONLSM 510 Image OptimizationB 40-051 e 07/98 4-894.7.2 Pinhole adjustment+ In all existing standard configurations, the pinholes have already been adjusted at the factory.These settings are taken over for active operation when a standard configuration is loaded.If you want to create a setting that differs from the standard configurations, adjust the pinholeas follows:Manual pinhole adjustmentThe position of the pinhole relative to the detector in terms of X-Y-Z coordinates contributes substantiallyto image optimization.Requirements to make pinhole position changes visible immediately: The image must be scanned by the continuous scan method. Select a fast scanning speed (see next section). Measurement with Average Number 1 only (no averaging of several measurements). On the Channel Settings panel (click on button in the "Scan Control" window),select the pinhole diameter so as to have the best possible image contrast (Airy Units 1.0). Click on the button of the main menu toolbar. This opens another, subordinate toolbar in the main menu.Fig. 4-98