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OPERATIONLSM 510 AnnexB 40-051 e 07/98 4-177 Clicking on the additional button moves the HRZ 200 to the zero position, while themotor focus moves into the opposite direction at the same time, i.e. the position of the object inrelation to the objective remains unchanged. This function is used to set defined initial conditions. The slider must normally be left in the default position 0. Calibration is required only ifthe examined image field is located clearly outside the center of the specimen carrier on the HRZ 200.Calibration is described in the next section.4.12.4.4 Additional information on the operationThe HRZ 200 fine-focusing stage is a high-precision, sensitive accessory for the LSM 510 from Carl Zeissand must therefore be treated carefully. High mechanical stress, such as the use of specimens weighingmore than 100 g or the application of pressure or knocks on the movable stage tongue, can result indamage and therefore in failure of the stage function.To be able to fully utilize the outstanding precision attainable with the fine focusing stage, anythingwhich could interfere with its operation, especially mechanical knocks and impact of the LSMcomponents, should be avoided. We would recommend you to always use the actively vibration-dampedKinetics stage (available as accessory under the order number 1007 508 or 1007 512) as the base for thesetup of LSM systems containing the HRZ 200 stage.The specifications of the stage are obtained only after a heating phase of approx. 30 minutes.Furthermore, the installation conditions for the LSM system must be observed.The maximum reproducibility (better than 40 nm) for moving to an absolute position in z is achieved byalways moving to the required position from below.Fine focusing is performed mechanically via an inclined position of the stage tongue. Therefore, thelifting range z at the location of the image field depends on the position of the HRZ in relation to theoptical axis. This means: if the user shifts the object on the microscope stage to the right via the HRZ200, the lift will be different from the one in the zero position of the stage (max. 200 μm) and also fromthe one after a shift of the stage to the left.If the LSM system is equipped with a motorized scanning stage, this shift is read back to x and the lift iscalibrated automatically if the zero position of the HRZ has been matched to the zero position of thescanning stage via an initialization run. For this, activate the dialog in the menu.Then position the scanning stage in such a way that the optical axis of the microscope corresponds tothe zero position of the HRZ, i.e. to the center of the specimen holder in the stage tongue. Then performinitialization by pressing the button. This step must be repeated after every new start of thesystem. Also see the notes on the operation of the motorized scanning stages.If the system is equipped with a manual microscope stage, the user has the option of performing thecalibration by entering the x shift in mm via the slider. |
