Zeiss LSM 510 Operating Manual

Also see for LSM 510 Inverted: Quick guide Operating manual User guide User manual Brief Operating Manual

INTRODUCTION TO LASER SCANNING MICROSCOPYPerformance Features of the LSM 510 LSM 5103-10 B 40-051 e 07/98(3) Transmitted-light illuminationa) The illuminator support contains a security circuit, which activates a shutter preventing laser light fromreaching the stand when the support is moved to back. A complementary shutter built-in the standprevents laser light from reaching the eye pieces during scanning mode.b) The illuminator support is equipped with a rotatable polarizer. The Axiovert description contains theadjustment for DIC mode during conventional observation.For scanning transmitted light DIC mode the polarizer in the transmitted light support works like ananalyzer and must be adjusted in such a manner, that direct laser light will be blocked.The conventional analyzer slider in the stand is not allowed be located in the beam path because of thelaser light already is polarized.c) On the illuminator support as an option there is mounted a LSM 5 software controlled switchingmirror fully motorized. Alternatively the light is directed to the LSM 5 T-light detector or enablesconventional transmitted-light observation.d) The focusing screen for conventional transmitted-light is located in a support in front of the halogenlamp housing.e) Further information to halogen lamp and condensers you will find in the Axiovert operating manual.(4) Reflected light fluorescenceAll Axiovert fluorescence accessories exceptional the reflector slider can be used.Further information you will find in the Axiovert operation manual.(5) Imaging opticsOptovar sliders are not usable.The analyzer for conventional DIC mode will be operated from the right side and is located just belowthe nosepiece.Use of sliders with auxiliary objects (473704/14) is not possible.(6) Photo equipmentThe stand doesn´t have an integrated SLR-port, but microscope cameras, as described in the Axiovertoperation manual, can be used.(7) TV adaptionThe TV port aside and the tubes can be used as described in the Axiovert operation manual.The TV interface side port or base port can only be used with TV adapters 44.