Zeiss LSM 510 Operating Manual

Also see for LSM 510 Inverted: Quick guide Operating manual User guide User manual Brief Operating Manual

INTRODUCTION TO LASER SCANNING MICROSCOPYOptical Diagram of the LSM 510 LSM 5103-6 B 40-051 e 07/983.3 Optical Diagram of the LSM 510 (Schematic)Fig. 3-2 Optical path, schematic (4-channel configuration)AOTF Acousto Optical Tunable FilterDBC Dichroic Beam CombinerDBS Dichroic Beam SplitterEF Emission FilterHAL Halogen LampHBO Mercury Vapor Short-Arc LampLSF Line Selection FilterMDBS Main Dichroic Beam SplitterNDF Neutral Density FilterVP Variable PinholePMT PhotomultiplierT-PMT Transmission-PhotomultiplierThe diagram above is a schematic representation of the LSM system.Laser light is focused onto the specimen through an objective in a diffraction-limited mode. Light emittedat the focal plane and at planes below and above it is directed via an XY scanner onto a main dichroicbeam splitter (MDBS), which separates the emissions from the excitation light. The fluorescences areseparated from each other by a series of dichroic beam splitters (DBS1 ... maximally DBS4) and directedto individual photomultipliers (PMT1 ... maximally PMT4).Fiber CouplerAOTFShutterEyepieceTube LensTube LensHBOObjectiveSpecimenCondensorT-PMTHALScanLens ScanneryXMirrorDBCPlateCollimatorLSF NDFMonitorDiodePinholeOpticsMDBSDBS2DBS1DBS3PMT3PMT4VP4EF4VP2EF2VP1EF1VP3EF3PMT2PMT1Ar-UV LaserAr/ArKr LaserHeNe LaserHeNe LaserUV FiberVIS FiberFiber CouplerAOTFShutterLaserModule VISScan ModuleMicroscope LaserModule UV