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OPERATIONLSM 510 Image Acquisition (Acquire)B 40-051 e 07/98 4-274.5 Image Acquisition (Acquire) In the main menu toolbar, click on . This opens another, subordinate toolbar in the main menu.Fig. 4-30+ For preparing and acquiring a scanning image, it is recommended to call up and use the toolsof the subordinate toolbar in succession from left to right. Laser setting. Conventional microscope setting. Configuring the optical system for the Scanning Mode. Setting of scan parameters. permits up to 10 areas within a frame to be defined and scanned. permits user-specific time series to be selected for the scan procedure. The function is used to bleach a defined, freely selectable area within the scanning field. Upon selecting you can set the focus (Z coordinate) and the Z step size between successiveslices. If the optional, motorized X/Y-stage is connected, the X and Y-positions of the sample can alsobe selected. With the , and buttons you can effect a PC-supported switching of the beampath. VIS for conventional microscopy through the eyepieces. TV conventional microscopy for observation by means of a TV camera. LSM Laser scanning mode.+ For the scanning process, the button in the toolbar subordinate to the "Acquire" itemmust be activated, and the silver slider on the microscope must be in the LSM position.Toolbar subordinate to the item |
