OPERATIONLSM 510 Quick startB 40-051 e 07/98 4-194.3.7 Z SectioningOnce you have set up your image as defined in the above section, you can collect a series of confocalimages through the different focal planes of your specimen.(1) Click on the button on the Scan Control menu.(2) If you have reduced the scan speed or have set image averaging, you should use the fast scanningmode to find the lowest and highest points of focus. These settings are made under Mode on theScan Control menu, or directly via the button.(3) Click on the button in the Z Settings panel.(4) Click on the Mark First/Last register in the Z Settings panel.Fig. 4-19