Zeiss LSM 510 Operating Manual

Also see for LSM 510 Inverted: Quick guide Operating manual User guide User manual Brief Operating Manual

OPERATIONLSM 510 Image Acquisition (Acquire)B 40-051 e 07/98 4-61Mark First/LastOptimum stack size can be obtained by manualfocusing during scan movement to the level wherethe stack should start. Proceed as follows Open the Mark First/Last register. Start the scanning process by clicking the button. Use focusing drive to set the focus position tothe required Z-plane and press Mark Firstbutton. Then focus on the opposite Z-plane and pressMark Last button.The XY-Cont Scan must be finished via before acquisition of Z Stacks can be startet via.The , and buttons allow afast overview of the first, middle and last scanningstep. Click on the button. Click on the button. Scanning process for stack recording will bestarted.Before the button is pressed, the followingsettings must be made and checked: Depending on the immersion medium used,the suitable correction value must beselected under Refr. Corr. This correctionvalue is the quotient of n/n’, n = refractiveindex of the sample and n’ = refractive indexof immersion medium.Fig. 4-62