Zeiss LSM 510 Operating Manual

Also see for LSM 510 Inverted: Quick guide Operating manual User guide User manual Brief Operating Manual

OPERATIONImage Acquisition (Acquire) LSM 5104-66 B 40-51 e 07/984.5.4.4 Time Series functionThe Time Series function offers the following options for the creation of a scanning image: Definition of break times between 0.1 ms and 10 hours. Determination of the number of steps from 1 to 10,000 for one scanning procedure. Setting of markers for a scanning procedure. Interruption of time control via break function, and start of the time function. Triggering of time series via: numeric input external trigger pulses time(1) Click on the and buttons in the LSM 510 tool bar. The "Time SeriesControl" window appears on the screen.(2) Set the relevant parameters for time control in the Start Series, End Series and Cycle Delayboxes.(3) Use the to buttons to set markers during the scanning procedure which will allowyou to evaluate interesting scanning images later.Note: "Time end" will finish time series even if you have created a program which would exceed thetime end. Bleach times will be added. No break is possible during bleaching. If you want to integrate a bleaching procedure in a time series, start must be triggered via"Start B". If a time series is interrupted before its programmed end, the programmed number of images willbe taken over in the database. However, only those images are stored which were created beforeinterrupting the time series. This is due to the fact that the original image parameters are to betaken over via the Reuse function.