Zeiss LSM 510 Manual

Also see for LSM 510: Quick start

OPERATION IN EXPERT MODE LSM 510 DuoScanCarl Zeiss Acquire Menu LSM 510 META DuoScan4-66 B 45-0021 e 03/064.5.3.8 Settings in the Lambda ModeThe Lambda Mode settings are only available ifthe LSM 510 contains the META detector. Withthis spectral module the overall emission from thesample is directed onto a wavelength-dispersiveelement and is imaged on a 32 channel detector.All 32 photomultipliers of the detector cover aspectral width of approximately 340 nm, a singlePMT covers a spectral range of 10.7 nm. In theLambda Mode, images, image stacks or timeseries can be recorded in a wavelength selectiveway.This new experiment is called Lambda Stack. Forthe acquisition of a Lambda Stack, thefluorescence signal of 8 PMTs out of the 32 can beread out at once. The settings of the beam pathfor the Lambda Mode scanning procedure withregard to the main dichroic beam splitter and theMETA detector settings are performed in theBeam Path and Channel Assignment panel.• Click on the Config Button in the AcquireSubordinate toolbar of the main menu.− The Configuration Control window opens.• Click on the Lambda Mode button, unless ithas already been activated.− The Beam Path and Channel Assignmentpanel for the Lambda Mode is opened.(1) Beam Path and Channel Assignment panelThe Beam Path and Channel Assignment panel displays the configuration of laser lines, the main(dichroic) beam splitter and the spectral range of the META detector to be covered.You can change the settings of this panel using the following function elements:Beam Path configurationActivation / deactivation of the excitation wavelengths (check box) and setting ofexcitation intensities (slider). Open the Laser Control window via the Laserbutton. The active laser lines are automatically displayed in the wavelength colorbar.Selection of the main dichroic beam splitter (HFT) through selection from therelevant list box.META detector slider Definition of number of PMTs out of the 32 PMT Detectors to fit the requiredspectral range.Fig. 4-47 Configuration Control window;Lambda Mode activated