Zeiss LSM 510 Manual

Also see for LSM 510: Quick start

LSM 510 OPERATION IN EXPERT MODELSM 510 META Acquire Menu Carl Zeiss03/06 B 45-0021 e 4-67Control buttonsClose Closes the Configuration Control window.Spectra The Spectra button opens the Detection Spectra & Laser Lines window (seepage 4-53).Laserline The Laserline button opens the Wavelength Switch Control window (seepage 4-54).Config The Config button permits existing track configurations to be loaded, storedunder any name, or deleted (see page 4-54).(2) Beam path - Excitation• On the Beam Path and Channel Assignment panel, click on the Excitation button.− This opens a dialog box of all available lasers with their wavelengths and their usable Acousto-Optical Tunable Filters (AOTF) attenuation.• To select the desired laser line, activate the check box for Line Active.• Use the Transmission [%] slider to set the utilizable laser intensity (recommendation: start at 50 %).− The transmittance of the Acousto-Optical Tunable Filter (AOTF) changes accordingly.• This allows you to adapt the laser intensity very sensitively to the job. Activate the check box for LineActive.− This activates the selected laser power for use. This is indicated by the Laser Power displayinglamps (status display green / gray).By clicking on the Excitation button you can check at any time which lasers are available foractive operation.If you deactivate Line Active, the laser wavelengths are deselected by means of the Acousto-Optical Tunable Filters (AOTF), i.e. these lasers change into standby status.If you interrupt your work with the LSM for a break, it is recommended not to switch theEnterprise and argon lasers off by hardware action, but to put them into standby status asdescribed.Excitation filters, emission filters, HFT main dichroic beam splitters and NFT secondary dichroicbeam splitters can be switched online, channels (PMT photomultipliers) only off-line.(3) Beam path - HFT main (dichroic) beam splitter• On the Beam Path and Channel Assignment panel, click on the HFT main dichroic beam splitters.− This opens a graphical pop-up window of all beam splitters available.• To select a beam splitter, click on the respective line of the list.− The selected beam splitter moves into the beam path.