Zeiss LSM 510 Manual

Also see for LSM 510: Quick start

LSM 510 3D FOR LSMLSM 510 META Functions Carl Zeiss03/06 B 45-0021 e 7-35Segment - AutomaticThe function carries out an automatic grey value segmentation by means of thresholding.Fig. 7-25The Automatic tab sheet of the Segment dialog window must be selected. Segmentation is especiallyused to generate binary regions. These are required for the measurement.The function calculates the two strongest local minimums in the histogram of the Input image sequence.These values are used for the discrimination. Only one channel of a multichannel sequence can beselected as Input. Output will always be a single channel sequence. The vertical scaling of the histogramcan be adjusted with the scroll bar at the right edge of the histogram. This setting has no influence onthe function.The Green and Blue/Red option buttons of the parameter Colour determine whether the voxels within(Green) or outside (Blue/Red) of the grey value interval [L, H] are displayed with the correspondingcolour.If Green is selected, the voxels within the selected interval are highlighted in green. The rest of the imageretains its original grey values. The voxels with the grey values Low and Low+1 are displayed in blue. Thevoxels with the grey values High and High-1 are displayed in red.If Blue/Red is selected, the voxels with grey values within the interval Low, High remain unchanged.Voxels with grey values less than Low are highlighted in blue; those with grey values higher than Highare highlighted in red.If the Invert option is selected, the grey values outside the defined interval will be segmented.If the option Binary is selected, then all grey values in the range from Low to High will be set to white(grey value 255 (4095)) in the Output image sequence, while all others will be set to black (grey value 0).If the option is not selected, the grey values within the selected interval remain unchanged, while thoseoutside the range will be set to black.