Zeiss LSM 510 Manual

Also see for LSM 510: Quick start

LSM 510 3D FOR LSMLSM 510 META Functions Carl Zeiss03/06 B 45-0021 e 7-33Segment - InteractiveThis function carries out a grey value segmentation by means of thresholding.Fig. 7-24The Interactive tab sheet of Segment dialog window must be selected.Segmentation is especially used to generate binary regions. These are required for the measurement.Two threshold values determine which grey value range of the Input image sequence is preserved and/ordeleted in the Output image sequence. Only one channel of a multichannel sequence can be selected asInput. Output will always be a single channel sequence.The vertical scaling of the histogram can be adjusted with the scroll bar at the right edge of thehistogram. This setting has no influence on the function.The thresholds Low and High are determined either by moving the borderlines in the grey valuehistogram or by the scroll bars underneath. Furthermore, the values for Low, Center and High can be setthrough entry in the corresponding fields.To move the lower (L) and upper (H) thresholds at the same time, move the vertical line in the grey valuehistogram or set the scroll bar (C).The Green and Blue/Red option buttons of the parameter Colour determine whether the voxels within(Green) or outside (Blue/Red) of the grey value interval [L, H] are displayed with the correspondingcolour.If Green is selected, the voxels within the selected interval are highlighted in green. The rest of the imageretains its original grey values. The voxels with the grey values Low and Low+1 are displayed in blue. Thevoxels with the grey values High and High-1 are displayed in red.