Zeiss LSM 510 Manual

Also see for LSM 510: Quick start

OPERATION IN EXPERT MODE LSM 510 DuoScanCarl Zeiss Image Optimization LSM 510 META DuoScan4-346 B 45-0021 e 03/06• Click on the icon, activate the 543 nmlaser line and click on Line Active . Ifrequired, deactivate other laser lines which arenot needed.• Use the Transmission slider to set the laserintensity to approx. 30 % at first.The Beam Path and Channel Assignment panel displays the current configuration loaded.The set laser intensity must be subsequently optimized for the current situation via theTransmission slider.For overlaying fluorescence and transmitted-lightimages, click on the ChD (Transmission) button inthe Beam Path and Channel Assignment panel.The transmitted light PMT photomultiplier will beactivated.Of course, all other transmitted light applicationslike− phase contrast− differential interference contrast (DIC)− polarization contrast (Pol)− darkfieldcan also be performed.For the generation of images in reflection,the main dichroic beam splitter must be a neutral-density filter.Standard equipment contains a neutral-densityfilter with a division ratio of 80 to 20 % (at543 nm).Fig. 4-323 Excitation panelFig. 4-324 Configuration Control window