Zeiss LSM 510 Manual

Also see for LSM 510: Quick start

INTRODUCTION TO LASER SCANNING MICROSCOPY LSM 510 DuoScanCarl Zeiss Optical Diagram of the LSM 510 LSM 510 META DuoScan3-4 B 45-0021 e 03/063.3 Optical Diagram of the LSM 510 and LSM 510 META (Schematic)Fig. 3-2 Optical path, schematic (4-channel configuration)AOTF Acousto Optical Tunable FilterDBC Dichroic Beam CombinerDBS Dichroic Beam SplitterEF Emission FilterHAL Halogen LampHBO Mercury Vapor Short-Arc LampLSF Line Selection FilterMDBS Main Dichroic Beam SplitterNDF Neutral Density FilterVP Variable PinholePMT PhotomultiplierT-PMT Transmission-PhotomultiplierThe diagram above is a schematic representation of the LSM system.Laser light is focused onto the specimen through an objective in a diffraction-limited mode. Light emittedat the focal plane and at planes below and above it is directed via an XY scanner onto a main dichroicbeam splitter (MDBS), which separates the emissions from the excitation light. The fluorescences areseparated from each other by a series of dichroic beam splitters (DBS1 ... maximally DBS4) and directed toindividual photomultipliers (PMT1 ... maximally PMT4).