Zeiss LSM 510 Manual
Also see for LSM 510: Quick start
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OPERATION IN EXPERT MODE LSM 510 DuoScanCarl Zeiss Display and Analysis of Images LSM 510 META DuoScan4-272 B 45-0021 e 03/064.13.17.3 Ortho - 2D DeConVolution FunctionThe 2D deconvolution function causes orthogonal projection enhancement through the computedcorrection of the resolution in the Z-coordinate.Image enhancement is only effective for the two projections of a fluorescence stack in the Ortho displayor for an XZ-scan in fluorescence, and is also only computed for this.• Activate the 2D DCV button in the Orthogonal Sections toolbar.If the Fast button is activated, calculation of the 2D-deconvolution (inverse DCV mode) is performedimmediately.The 2D DCV settings button can only be activated if a licence for the 3D DCV option has been purchased.Otherwise this button is grayed.• Click on the Settings button. The 2D Deconvolution window is opened.The 2D Deconvolution window contains the Deconvolution panel with the two tabs Method andPSF.(1) Method tabThe Method tab enables you to choose betweenthe calculation methods Inverse and Iterative.For more details of explanation of deconvolutionand the calculation methods see 3DDeConVolution (page 4-170).In the Inverse method, the Restoration Effectslider can be used to set the signal-to-noise ratiobetween Weak (low noise) and Strong(pronounced noise).Activation of the Auto detect check box starts aroutine for the automatic determination of thenoise level in the entire image part of the Z Stack.If Auto detect is enabled, the Restoration Effectslider is disabled.The Iterative method permits (in addition to the parameters of the Inverse method) the maximumnumber of iterations to be entered between 1 and 200 under Maximum Iterations and the Auto Stopfunction to be activated / deactivated. The Auto Stop function interrupts the calculation depending onthe set image improvement (delta between last but one and last cycle in %), no matter whether the valueunder Maximum Iterations has been achieved or not.Fig. 4-267 Method tab |
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