Zeiss LSM 510 Manual

Also see for LSM 510: Quick start

LSM 510 OPERATION IN EXPERT MODELSM 510 META Macro Menu Carl Zeiss03/06 B 45-0021 e 4-195Orientate panelFind Focus button The objective chosen in the Multiple Profiles Parameters... window isperformed and a fast line scan over Z is acquired. The Z range of this line scanis 0.5 mm, the single Z step 300 nm. The stage Z Position is set to the height ofthe major maximum intensity position.Find Gain button When clicking on the Find Gain button a Find procedure as described on page4-72 is performed on the current Z slice.Z-Settings panelZ Range [μm] This parameter defines the total Z height a Multiple Profile and/or a MultipleStack is performed. Z range values could be changed between 20 microns and4 mm (if working distance of the objective will permit this, otherwise an errormessage appears on the screen.Because in Multiple Profile Scan the single Z step could only bevaried from 100 … 600 nm and the maximum number of Z slices islimited to 2000, the overall Z travel distance of the stage is also limitedto 200 … 1200 μm, respectively.Sample Center [%] This button defines whether the actual Z position of the stage is rather placedon top or on the button of the materials surface.Multiple Profile panelProfiles The number of single profiles which has to be acquired, can be varied between1 and 35. (Depending on the overlap total maximum size is restricted to lessthan 32k).Total Profile Length The calculated total profile length is displayedMultiple Stack panelStacks in X The number of stacks in X dimension can be varied between 1 and 32.Stacks in Y The number of stacks in Y dimension can be varied between 1 and 32.Multiple Profile buttonIf you click on the Multiple Profile button, the stage moves out of the center position of the sample andis moved to the left-hand side to perform a first single profile. After that an interim image is performedbefore and after the next step of the motorized XY stage. A cross correlation of the two profiles (Z) andthe two (not displayed) images (lateral) is performed to assure an accurate profile match online duringthe experiment.If you acquire the Multiple Profile Scan in Range Indicator palette the results of thecorrelation could directly be observed as "blue boxes".