Zeiss LSM 510 Manual

Also see for LSM 510: Quick start

OPERATION IN EXPERT MODE LSM 510 DuoScanCarl Zeiss Display and Analysis of Images LSM 510 META DuoScan4-288 B 45-0021 e 03/064.13.22 Display - 2.5 DThis function allows to− display the two-dimensional intensity distribution of an image in an pseudo 3D mode− show the intensity values in profile, grid or filled mode− show separate distribution for each channel in a multi channel imageThe 2.5 D button can also be used online during scanning.• Click on the 2.5 D button.− The Pseudo 3D toolbar is displayed.The settings of Slice apply. The settings of Chan, Zoom, Contr and Palette are not applied.Click on 2.5 D will display the Pseudo 3D toolbar. Any changes done with this toolbar are effectiveimmediately. The content of the overlay plane is temporarily deleted while the toolbar is displayed.The viewing plane of the Image Display window can be rotated, tilted either directly with the mouse orby the scroll bars on the right-hand side and the bottom of the Image Display window.4.13.22.1 Direct Setting in the Image• Click in the image and hold down the mouse button. The perspective is changed by moving themouse button in horizontal or vertical direction.4.13.22.2 Setting via Scrollbars• Move the horizontal scrollbar to rotate the image around the vertical axis. The rotation angle isdisplayed in the yellow display box.• Move the left vertical scrollbar to rotate the image around the horizontal axis. The rotation angle isdisplayed in the yellow display box.The intensity scale can be varied by the scroll bar on the right-hand side of the Image Display window:• Moving the right vertical scrollbar enables you to expand or to compress the intensity scale of theimage, while the expansion of this intensity axis ranges between 10 % and 100 % of the X-scale size.