Zeiss LSM 510 Manual

Also see for LSM 510: Quick start

OPERATION IN EXPERT MODE LSM 510 DuoScanCarl Zeiss 3D View Menu LSM 510 META DuoScan4-170 B 45-0021 e 03/064.7.4 3D DeConVolution (DCV)The 3D Deconvolution option is used for the resolution enhancement of fluorescence image stacks.When a three-dimensional object is reproduced by an optical system the resulting image of the objectdoes not correspond exactly to the object's actual form. The image of the object is "distorted" as itpasses through the optical system. In physical terms the actual object is convolved by the optical system'sPoint Spread Function (PSF).The Point Spread Function describes how the light of a point object is distorted by the optical system.This "convolution" makes the image appear grainy and structures in the image seem blurred. This effectis most prominent in the axial (Z-)direction as each lens is optimized for the two-dimensional image of theobject.If the PSF is known it is possible to use mathematical algorithms to undo this distortion. The image of theobject is deconvolved using the PSF and the actual form is reconstructed:The effect of 3D deconvolution can be demonstrated impressively on objects with a known form. As arule fluorescent beads are used for this purpose. The following figure shows the 3D deconvolution of animage stack with a fluorescent bead with a diameter of 1 μm.Fig. 4-163 Point Spread Function (PSF)Fig. 4-164 Point Spread Function (PSF)