LSM 510 ANNEXLSM 510 META Monitor Diode Carl Zeiss03/06 B 45-0021 e 8-23Amplifier GainThe diode signal is set to the right range (gray levelbetween 50 and 200 - 8 bit image / 750 and3500 - 12 bit image) with the help of gray filtersand amplifier gain. The use of a lower filter densityshould be prioritized against the use of a high gainvalue.The value of the amplifier gain of both channels(PMT and diode) should be set to one, if possible.Because of an increasing amplifier noise, parallel tothe gain factor, a gain value of more than 2 shouldbe avoided. The most important thing is to avoidpixels below the zero level and beyond themaximum range respectively.Fig. 8-9 Scan Control window

Zeiss LSM 510 Manual