Zeiss LSM 510 Manual

Also see for LSM 510: Quick start

8 03/06Configuring the beam path and lasers• Click on the LSM button in the Acquiresubordinate toolbar for laser scanning.Choosing the configurationSingle Track− Use for single, double and triple labeling;simultaneous scanning only− Advantage: faster image acquisition− Disadvantage: cross talk between channelsMulti Track− Use for double and triple labeling; sequentialscanning, line by line or frame by frame− Advantage: when one track is active, onlyone detector and one laser is switched on.This reduces cross talk.− Disadvantage: slower image acquisition• Click on the Config button in theAcquire subordinate toolbar toopen the Configuration Controlwindow.The Configuration Control windowappears (Fig. 12).Setting for single track configuration in Channel Mode• Select Channel Mode if necessary (Fig. 12).• Click on the Single Track button in the Configuration Control window (Fig. 12).• Click on the Descanned button if necessary.The Beam Path and Channel Assignment panel of the Configuration Control window displays theselected track configuration which is used for the scan procedure.• You can change the settings of this panel using the following function elements:Activation / deactivation of the excitation wavelengths (check box) and setting ofexcitation intensities (slider). Open the Laser Control window via the Laser button.Selection of the main dichroic beam splitter (HFT) or secondary dichroic beam splitter(NFT) position through selection from the relevant list box.Selection of an emission filter through selection from the relevant list box.Activation / deactivation (via check box) of the selected channel (Ch 1-4, monitor diodeChM, META detectors ChS1-8, transmission ChD) for the scanning procedure byassigning an existing color icon or defining a new one.Fig. 12 Configuration Control window forSingle Track