Zeiss LSM 510 Manual

Also see for LSM 510: Quick start

OPERATION IN EXPERT MODE LSM 510 DuoScanCarl Zeiss Acquire Menu LSM 510 META DuoScan4-72 B 45-0021 e 03/064.5.4.1 Open / Close the Scan Control Window• Click on the Scan button in the Acquire subordinate toolbar of the Main menu.− This opens the Scan Control window, which shows all lasers connected to the system.• Click on the Close button to quit the Scan Control window.The following main function buttons are available in the Scan Control window:Generally available buttonsMode button When the button is activated, the following panels are available for thesetting of the scanning parameters for the line and frame modes:Objective Lens, Image Size & Line Step Factor, Speed, Pixel Depth,Scan Direction & Scan Average and Zoom, Rotation & Offset.Channels button When the button is activated, the Channel Settings andExcitation of Track ... panels are available for the setting of thechannels and the laser excitation.Spot button Activates the Spot scan mode.Line button Activates the Line scan mode.Frame button Activates the Frame scan mode.Use ROI button Activates the scanning procedure only within a ROI (region of interest) tobe defined first.Z Stack button Activates the Z Stack scan mode, display of additional buttons on theright-hand side of the Scan Control window.Z Settings button When the button is activated, the Z Settings panel is available for the Z-scan parameter definition. The Z Stack scan mode must be active.Close button Closes the Scan Control window.New button Opens a new Image Display window.Find button Automatic optimization of image brightness and contrast. The settingsfor the Find function can be varied as required using the Maintainmenu, Set Find (see page 4-242).Fast XY button Continuous scan with high speed. This function should be used to alimited extent and only for a short period of time. Fast XY switchestemporarily to 512 x 512 frame size.Single button Single scan (named Start in the Z Stack mode).Stop button Stops the current scan procedure, no matter in which window thebutton is pressed (also see the Time Series Control and Bleach Controlwindows).Cont. button / Finish button Continuous scan (not available in Spot Scan mode and Z Stack mode).If you select the option Frame for Mode and the option Continuous forNumber in the Pixel Depth, Scan Direction & Scan Average panel,the Finish button is displayed instead of the Cont. button. In this case,continuous averaging is performed when you have started the scan. Ifyou click on the Finish button, the scan/averaging process is stoppedafter the scan of the current image has been completed.