Zeiss LSM 510 Manual

Also see for LSM 510: Quick start

INTRODUCTION TO LASER SCANNING MICROSCOPY LSM 510 DuoScanCarl Zeiss Performance Features of the LSM 510 … LSM 510 META DuoScan3-6 B 45-0021 e 03/06Selection of the specimen detail of interest for zooming is fast and convenient, and the zoomed image isautomatically centered. This saves the job of specimen centration with the microscope stage.Using a bi-directional scanning facility will double the scanning rate to approx. 5 frames/sec (at 512 × 512pixels); if two different laser wavelengths are used for the two scanning directions (wavelength 1 for left-to-right, and wavelength 2 for right-to-left scanning), two fluorochrome dyes can be viewed anddocumented in a quasi-simultaneous mode. This will prevent cross talk between detection channels.The LSM 510 and LSM 510 META can be combined with a second scanner, the LSM DuoScan. Any of theabove mentioned microscopes can be used. The LSM DuoScan can be equipped either with a UV Laser ora 405 nm laser both also together with a 488 and/or 532 nm laser. The software allows to simultaneouslyimage and bleach/manipulate a sample. The usefulness of this option is dependent on the sample.3.4.2 Microscope Equipment of the LSM 510 and LSM 510 META SystemThe LSM 510 or LSM 510 META system is equipped either with the inverted Axiovert 200 M BP or SPmicroscope or with the upright Axio Imager.Z1, Axio Imager.M1 or Axioskop 2 FS MOT microscopes.Only the differences from the delivered operating manual "Axiovert 200 M" will be explained here.(1) Standa) The motorized objective nosepiece 5× H DIC is firmly fixed to the stand, where no operating elementscan be found for the nosepiece. Operation will be performed via LSM 5 software control. The "Restrictionof the nosepiece height to protect the objectives during motorized objective change" is inactivated. Thenosepiece will be moved down automatically before each motorized objective change.b) The reflector mount is motorized and provided with the Axiovert 200 M reflector turret. The reflectorturret has 5 positions: One transmitting light position, which is identical to the LSM position, and fourfurther positions for fluorescence filter sets (reflector modules). If you want to use more than fiveconventional fluorescence filter sets, it is advisable to use a further reflector turret. When changing thereflector turret position you must make sure that the turret will click into position, since otherwise theimage area will be cut. If the system is equipped with the LSM DuoScan up to three positions (dependingon the laser equipment of the LSM DuoScan) are equipped with fixed beam combiners. Two positions ofthe reflector turrent can be equipped with conventional fluorescence filter sets.c) The stand has a motorized focusing drive (fine coarse). Sensitivity of the focusing drive is adjusted tothe delivered objectives by the manufacturer. If you want to use other objectives, sensitivity andparfocality can be adjusted via the Axioset program.d) The stand features an integrated power supply for the internal motors and stand electronics. Thepower supply can be switched on at the right side of the stand. External power supply units will be usedfor the mercury vapor short arc lamp.e) The analyzer slider for conventional DIC methods will be operated from the right side and is locatedjust below the nosepiece.When the rod is pushed in, the analyzer is located in the beam path. In the LSM-mode, the analyzer mustnot be located in the beam path, and the analyzer rod must be pulled out.(2) Specimen stages and fine focus drivesa) Mechanical stageThe stage with coaxial drive must be mounted on the right side of the stand.b) Scanning stagec) Piezo objective focus drive