OPERATION IN EXPERT MODELSM 510DuoScanCarl ZeissProcess MenuLSM 510 METADuoScan4-150B 45-0021 e03/064.6.12.3Single Wavelength Dyes – Offline Calibration•Subtract background/autofluorescence image from raw images to obtain.•Perform equation- or titration calibration (compare F with a calibration curve→titration calibration orput F values in calibration formula).Fig. 4-138Ion Concentration window