Zeiss Lightsheet Z.1 Operating Manual

Contents

CHAPTER 4 - SYSTEM OPERATIONCarl Zeiss Contents Lightsheet Z.12 000000-1790-528 02/20133 LEFT TOOL AREA AND HARDWARE CONTROL TOOLS ................................... 373.1 Locate Tab ................................................................................................................... 373.1.1 Locate Capillary ............................................................................................................. 383.1.2 Locate Sample ............................................................................................................... 383.1.3 Specimen Navigator Tool................................................................................................ 393.1.4 Incubator Tool ............................................................................................................... 403.2 Acquisition Tab ........................................................................................................... 413.2.1 Experiment Manager...................................................................................................... 423.2.2 Action Buttons............................................................................................................... 433.2.3 Multidimensional Acquisition Settings............................................................................. 443.2.4 Setup Manager – Laser................................................................................................... 463.2.5 Setup Manager – Light Path ........................................................................................... 473.2.5.1 Track Definition ............................................................................................................. 483.2.5.2 Transmitted Light Lamp Control ..................................................................................... 493.2.5.3 Laser Control ................................................................................................................. 493.2.5.4 Incubator ....................................................................................................................... 493.2.5.5 Detection Optics Display................................................................................................. 503.2.5.6 Laser Blocking Filter Selection ......................................................................................... 503.2.5.7 Emission Selection Filter Selection ................................................................................... 503.2.5.8 Channel Selection .......................................................................................................... 513.2.6 Specimen Navigator Tool................................................................................................ 523.2.7 Acquisition Parameter – Acquisition Mode ...................................................................... 533.2.8 Acquisition Parameter – Channel .................................................................................... 573.2.8.1 Defining Channels and Tracks ........................................................................................ 573.2.8.2 Defining Detector and Illumination Settings .................................................................... 613.2.8.3 Defining Display Options ................................................................................................ 643.2.9 Acquisition Parameter – Incubator .................................................................................. 653.2.10 Multidimensional Acquisition – Z-Stack ........................................................................... 663.2.10.1 Performing a Z-Stack Using First/Last Mode..................................................................... 663.2.10.2 Performing a Z-Stack Using Center Mode ....................................................................... 683.2.11 Multidimensional Acquisition – Time Series ..................................................................... 693.2.11.1 Interval Time .................................................................................................................. 713.2.11.2 Marker .......................................................................................................................... 723.2.11.3 Start .............................................................................................................................. 743.2.11.4 End ............................................................................................................................... 753.2.12 Multidimensional Acquisition – Multiview-Setup ............................................................. 763.2.13 Multidimensional Acquisition – Information on Experiment ............................................. 813.3 Processing Tab ............................................................................................................ 823.3.1 General Structure of the Processing Tab ......................................................................... 823.3.2 Processing – Maximum Intensity Projection ..................................................................... 863.3.3 Processing – Color-coded Projection ............................................................................... 873.3.4 Processing – Image Calculator ........................................................................................ 883.3.5 Processing – Average ..................................................................................................... 903.3.6 Processing – Filter .......................................................................................................... 913.3.7 Processing – Linear Unmixing ......................................................................................... 94