Zeiss Lightsheet Z.1 Operating Manual

Contents

CHAPTER 4 - SYSTEM OPERATIONCarl Zeiss Center Screen Area / Image Containers - Display and Image Analysis Lightsheet Z.1214 000000-1790-528 02/20134.8.7 Flying ModeThe Flying mode is an interactive way to explore three-dimensional data. By pressing the mouse wheelyou can fly into and through a dataset. Moving the mouse left / right and up / down controls the flyingdirection. Pressing the mouse wheel and the right mouse button at the same time allows for flyingbackwards.Once the Flying mode is activated, the rotation of the data switches modes: Whereas normally thedata turns around an imaginative point in the center of the dataset, in the fly mode, the data turns(if moved interactively with the mouse once one stops flying) around an imaginative point "behind"the operator. Pressing the button switches back into the normal mode.If a wheel-mouse is not available, pressing the button turns the left mouse button into the Flyingbutton.The Flying mode is only available in graphics card accelerated render modes.4.8.8 3D Rendering Settings in VisArtDepending on the render mode selected, thefollowing adjustments can be applied to thedatasets:All settings can be saved and re-applied to otherdatasets by using the buttons.− Threshold specifies a lower threshold as apercentage of the gray values displayed. Thisdecides which data enter the renderedimage. If one fluorescence channel containsa homogeneous fluorescence signal withhigh signal strength, one would set thethreshold higher in order to "protect"signals from weaker channels from beingquenched.− Ambient describes a diffuse, non-directed light source, which lets both bright and dark structuresappear darker or brighter in an equal fashion (values on a scale between 0 and 100).− Specular sets a directional brightness (values on a scale between 0 and 100). Influences thedifferences between dark and bright structures, which are strongest at a value of 0.− Shininess changes the surface gloss (on a scale between 0 and 100).− Ramp controls the degree of the transition from completely transparent to completely opaque.− Maximum influences the degree of opacity.The settings are combined into a histogram . The x axis corresponds to the gray values andthe y axis to the degree of opacity. You can also use the mouse to interact with the histogramcurve.Fig. 320 3D Rendering window (e.g. SurfaceAdvanced rendering mode)