Zeiss Lightsheet Z.1 Operating Manual

Contents

CHAPTER 4 - SYSTEM OPERATIONCarl Zeiss Center Screen Area / Image Containers - Display and Image Analysis Lightsheet Z.1224 000000-1790-528 02/2013Histogram functions:Skip Black and Skip White tick boxes: Ignore black pixels (gray value 0) andIgnore white pixels (gray value 255 or 4096) in the histogram.Step slider and spin box: Sets the number of intensity steps ("trays") of thehistogram display. Step 1 corresponds to 256 intensity steps, Step 64 to 4intensity steps (for 8 bit images). Reduction is made by averaging.Scaling pull down menu: Sets the histogram graph scaling of the AbsoluteFrequency to either dynamic (adjusting to the maximum value) or fixedShow Table tick box: A table of the intensity values is shown in the ImageDisplay window.Show Statistics tick box: Displays statistical parameters (Mean Intensity,Standard Deviation, Number of Pixels, and Size of Area) in an additionaltable. Area measurements of very small areas (<10 pixels) give onlyapproximate values.Show Image tick box: Shows the image in the Image Display window withthe histogram graph.The Cut Mask tool creates a new image document which sets every pixeloutside ROIs to Zero. Within the ROIs, only the pixels with values betweenlower and upper threshold are transferred to the new image.Tables can always be saved or copied to the clipboard by right-mouse clicking on the tabledisplay!Histogram functions and Overlays:The functionality of the Graphics View Option control block fully applies to the Histo View Type. If aROI is marked/selected in the Histo View Image display, only this ROI will be taken into account for thehistogram display, the table, and the statistics. If there are several ROIs, but none is marked/selected, thehistogram display, the table, and the statistics analyze all the pixels included in all ROIs.