Zeiss Lightsheet Z.1 Operating Manual

Contents

CHAPTER 1 - HARDWARELightsheet Z.1 User Interfaces Carl Zeiss02/2013 000000-1790-528 433.8 Switch Incubation ON and OFF• Before you turn ON the incubation, all incubation components you wish to use during the imagingsession must be attached to the Lightsheet Z.1 system before starting ZEN software.• Set the "Incubation" switch on the remote control to the ON position. Make sure that all incubationcomponents are switched on.• Once ZEN is running you can detach and reattach components.• To turn OFF the incubation, uncheck the corresponding checkboxes in the Incubator tool window(see CHAPTER 4 SYSTEM OPERATIONl) or deactivate the incubation components by setting the"Incubation" switch to the OFF position.Be aware that all incubation components will continue working with the in Incubator tool windowspecified parameters after ZEN has been shut down or stopped working. This will keep your samplealive under precisely defined conditions, even if ZEN stops unexpected during an experiment.3.9 Illumination and Detection OpticsIn the Lightsheet Z.1, one or a pair of illumination optics plus one detection optic are always required.We recommend the following combinations:Lightsheet Z.1 illuminationoptics 5× / 0.1Lightsheet Z.1 illuminationoptics 10× / 0.2Lightsheet Z.1 detection optics5× / 0.16 yes noLightsheet Z.1 detection optics20× / 1.0 no yesLightsheet Z.1 detection optics40× / 1.0 no yesLightsheet Z.1 detection optics63× / 1.0 no yes