Zeiss Lightsheet Z.1 Operating Manual

Contents

CHAPTER 1 - HARDWARELightsheet Z.1 User Interfaces Carl Zeiss02/2013 000000-1790-528 17Step 4 is the final step in which the alignment forall filters and detection module channels isperformed. A progress bar is present at the bottomof the window. When the procedure is finished theFinish button becomes available, press it to saveall adjustments and leave the wizard. This text isdisplayed: “Detector Alignment for all availablefilters in progress, please wait.”If you still encounter pixel shifts between the twochannels after the Automatic Camera Alignmenttool, you can fine tune settings using the ManualDetector Alignment tool, see section 3.2.3 Adjustthe Grating Focus for the Automatic orManual Detector Alignment Tool.3.2.2 Adjustment – Manual DetectorAlignmentThis adjustment is necessary for one or twodetection modules when you recognize a pixel shiftbetween channels, or when the zoom is notcentered.The Automatic Detector Alignmentshould always be performed first since itmight already solve the problems.Furthermore it defines the settings for thecombination Channel 1 (Cam1) andposition 1 of the Emission Selection Filter,which should be used as the referenceduring the Manual Detector Alignment.The Zoom in the Acquisition tool windowis fixed to 1 while performing the ManualDetector Alignment.Fig. 9 Automatic Detector Alignment,third stepFig. 10 Manual Detector Alignmentwindow