Zeiss Lightsheet Z.1 Operating Manual

Contents

CHAPTER 1 - HARDWARELightsheet Z.1 User Interfaces Carl Zeiss02/2013 000000-1790-528 19Use the sliders or the input box with arrows of Cam 1 X and Cam 1 Y (for Channel 1, Cam1) or Cam 2 Xand Cam 2 Y (for Channel 2, Cam 2) to move the previously chosen structure of the grating to overlaywith the cross. Finish this step by pressing the Store Current Settings button.Repeat the procedure for all desired Emission Selection filters and channels, always using the crossmarker as the reference.It is helpful, especially after an Automatic Detector Alignment has been performed, to use the ProfileView tab in order to evaluate if the image of Cam 1 and Cam 2 overlay. While the grating iscontinuously imaged, press the Profile View tab and draw a line or an arrow poly-line on the grating(Fig. 13). Use the sliders or the input box with arrows of Cam 1 X and Cam 1 Y (for Channel 1, Cam1) orCam 2 X and Cam 2 Y (for Channel 2, Cam 2) to move the lines of the grating to overlay each other.You can leave the Manual Detector Alignment by pressing the Close button at the bottom of thewindow.3.2.3 Adjust the Grating Focus for the Automatic or Manual Detector Alignment ToolDuring the Automatic Detector Alignment tool wizard and the Manual Detector Alignment tool agrating is brought into the light path which can be imaged on each channel. When the grating is out-of-focus, you need to refocus it for the relevant channel. For checking the focus, it helps to zoom into theimage using the Zoom function and look at the center cross within the square (Fig. 12) If the whiteportions within the inner most circle are recognizable, the grating is in focus.To adjust the focus of the grating start the Manual Detector Alignment by pressing the button in theAdjustment tool window. The grating will be brought into the beam path and an image is generatedand displayed in the image container of the main software.Fig. 13 Manual Detector Alignment, Grating, Profile view tab