Zeiss Lightsheet Z.1 Operating Manual

Contents

CHAPTER 1 - HARDWARECarl Zeiss User Interfaces Lightsheet Z.134 000000-1790-528 02/20135. Take the capillary by the glass portion (avoiding the plunger) with the agarose embedded specimeninside and carefully place it into the center of the clamp screw sample holder top.6. Push the capillary within the holder downwards until the color-coded capillary marking becomesvisible (Fig. 26/6 right hand side).Newly bought sleeves can be slightly tight at their slit opening ends so that in can be difficult topush the capillary within the holder. In this case take the capillary with matching plunger and insertit carefully into the corresponding sleeves at their non-slit endings. Make sure that the slits pointoutwards. Position the capillary surrounded by the sleeves into the sample holder stem and placethe clamp screw. You can proceed with step 6).7. Tighten the clamp screw. Take the sample holder disc (Fig. 26/1) with the protruding side withpositioning notch facing you. Carefully position the capillary stem holder with specimen into thesample disc holder until the ball bearing click-position is felt.8. Before taking the capillary out (chapter 2.6), retract the sample back inside the capillary glass viausing plunger.9. For removal, loosen the clamp screw and carefully pull the capillary through the stem sleeve byusing the glass end nearest the color-coded marking.10. Unscrew the clamp screw completely and remove the sleeves by using the ejection tool (Fig. 26/7)for the capillary sleeves removal.The sleeves as well as the glass portion of the capillary can be cleaned with a lint-free cloth moistenedwith water.3.5.2 Assembly of Sample Holder for Syringes− Place the sample holder disc for syringes (Fig. 27/1) so the flat side is facing you.− Take the adapter ring (Fig. 27/2) and place it into the center of the sample holder disc with thelarger diameter of the ring facing you.− Pick up the sample disc holder with adapter ring in one hand. Take the syringe carrying yourspecimen (Fig. 27/3) and insert it completely into the sample disc holder (avoiding touching theplunger).− Touching the syringe body and turn clock-wise to position the syringe body into the two clamps(Fig. 27/6).