Zeiss Lightsheet Z.1 Operating Manual

Contents

CHAPTER 4 - SYSTEM OPERATIONLightsheet Z.1 Left Tool Area and Hardware Control Tools Carl Zeiss02/2013 000000-1790-528 125Once the settings are complete press Apply to obtainthe trajectories of the single molecules (Fig. 189).3.3.15 Processing – Structured IlluminationLicensing Instrumentstandard  optional LSM  ELYRA Lightsheet Z.1Tool accepts data recorded in SIM mode on ELYRA. In data sets containing othertypes of image data as well ("mixed mode" files), this tool selectively processes theSIM data. "Experimental PSF" (requires Z-stacks of point-like objects) and "FourierTransform" accepts input other than SIM data as well. See full description of tool setfor details.The Processing Structured Illumination tool works exclusively for data acquired in the SIM imagingmode. It subdivides in 5 subcategories (Fig. 190):− Structured Illumination: calculates the high resolution image from the set of structured images− TIRF filtered: Will apply the TIRF sectioning to the SIM image if the TIRF was added as the lastframe.− Experimental PSF: Calculates the PSF from an image obtained with sub-resolution beads.− Modulation contrast: Determines the modulation contrast from an image with beads to serve asa diagnostic tool− Fourier transform: Calculates the spatial frequencies and displays them in an image. No phaseinformation will be available.Fig. 189 Image with trajectories of singlemolecules. The selected trajectory isboxed