Zeiss Lightsheet Z.1 Operating Manual

Contents

CHAPTER 1 - HARDWARECarl Zeiss Index Lightsheet Z.152 000000-1790-528 02/20134 IndexAAutomatic Detector Alignment ...................... 15CCapillary color coding .................................... 33Capillary size ................................................. 33Customized Sample Chamber .......................... 9DDetection ModulesAdjust Focus of Grating ............................. 19Alignment ................................................. 13Cable Connections "PCO.Edge" ................ 22Cable Connections "Standard" .................. 23Deinstallation............................................. 10Dismounting .............................................. 12Installation ................................................. 10Mounting .................................................. 12Registration ............................................... 13Detection optics ............................................ 43Exchange................................................... 46Serial number ............................................ 46Detector Alignment ....................................... 15Detector Recognition ..................................... 15EErgoDrive Operating Panel ........................... 6, 7IIllumination opticsExchange................................................... 45Illumination Optics ........................................ 43ImagePixel shift ................................................... 15Zoom not centered .................................... 15Image Area ................................................... 44IncubationCO2 -Module .............................................. 39Cover ........................................................ 30Heating Components ................................. 37Heating Device Humidity S1 ....................... 41Heatingblock ............................................. 37Installation of Incubation Modules .............. 37Peltier Block S ............................................ 38Registration of modules ............................. 41Temperature sensor ................................... 30Tempmodule CZ-LSFM ............................... 38MManual Detector Alignment ...........................17PPlunger reference number ..............................33RReflector Turret for Emission SelectionDatabase configuration...............................49Exchange ...................................................47Reflector Turret for Laser Blocking FilterExchange ...................................................49Push and click filter exchange .....................50Rotate around Center of image ....................... 7SSample ChamberAssembly....................................................24Assembly of Sample Chamber Body ............27Assembly of Sample Chamber Dove Tail Slide..............................................................27Assembly of Sample Chamber Windows .....24Blind Plugs .................................................29CO2 incubation connection .........................29Cover .........................................................30Customized ................................................. 9Detection optic window..............................25Drain Connector .........................................29Incubation Accessories ................................30Inserting .....................................................31Luer-Lock Connectors .................................29Removing ...................................................31Syringe based perfusion ..............................29Temperature sensor ....................................30Sample HolderAssembly....................................................32Capillary .....................................................32Inserting in System .....................................35Plunger ......................................................32Removing from System ...............................35Sleeves for Capillary ....................................32Syringe .......................................................34Specimen Navigator tool ................................. 7