Zeiss Lightsheet Z.1 Operating Manual

Contents

CHAPTER 1 - HARDWARECarl Zeiss User Interfaces Lightsheet Z.124 000000-1790-528 02/20133.3 Assembly of the Sample Chamber− Wear powder-free gloves to avoid fingerprints on the cover slips as well as to protect your handsfrom sharp edges.3.3.1 Assembly of the Sample Chamber Windows− Assemble the three sample chamber body windows (for the illumination optics on the sides andthe front chamber window for the overview camera with LED white illumination) using thefollowing steps:1. Take the sample chamber body (Fig. 18/1) and lay it onto the table so that one of the three smallerwindows are facing you.2. Using a forceps or gloved hands, remove one clean circular 18 mm cover slip; place cover slip intothe empty sample chamber body window so it fits into the smallest groove (Fig. 18/2) – you willneed a fine forceps.3. Now place the O-ring (18 mm O-ring, black) into the corresponding groove (Fig. 18/3). Make surenot to disturb the positioning of the cover slip.4. Take the silver illumination adapter ring (Fig. 18/4) and lay it into the window making sure the flatside with the four pinholes is facing you.5. Using the sample chamber window tool (Fig. 18/5), position the circular pins into the pinholes andturn clockwise until finger-tight. Adapter should be flush with the sample chamber body.1 Sample chamber body2 Cover slip3 O-ring (18 mm O-ring, black)4 Illumination adapter ring5 Sample chamber window toolFig. 18 Assembly of the three sample chamber windows for illumination optics and overviewcamera