Zeiss Lightsheet Z.1 Operating Manual

Contents

CHAPTER 1 - HARDWARELightsheet Z.1 User Interfaces Carl Zeiss02/2013 000000-1790-528 293.3.3 Insertion of the Drain Connector, Luer-Lock Connectors and Blind Plugs− Turn the assembled sample chamber body with dove tail slide so that the dove tail slide is on thetable with the grip facing you (Fig. 22/1).− Take the grey drain connector and screw this into the upper left hole of the sample chamber bodyuntil finger-tight (Fig. 22/6). This will guide excess liquid to the drain.− Take a Luer-Lock-connector (white) and screw it into the lower right corner of the sample chamberbody (Fig. 22/4). The Luer Lock connectors (white) are used to fill or perfuse the sample chamberwith liquids and / or gasses.− For CO2 incubation, the Luer-Lock connector must be positioned at the upper right corner of thesample chamber (Fig. 22/2). For syringe based perfusion one can use any of the three lower screwholes. The lower right corner screw hole is recommended for convenience (Fig. 22/4).− For perfusion pump use the right side bottom screw hole for the perfusion input and either of thefront lower corner screw holes for the perfusion output.− Close all remaining sample chamber screw hole openings by inserting the black blind plugs(Fig. 22/3; Fig. 22/5).1 Sample chamber body with dove tail slide2 Luer-Lock-connector (white); position recommend for CO2 incubation3 Blind plug (black)4 Luer-Lock-connector (white); position recommend for syringe based perfusion5 Blind plug (black)6 Drain connector (grey)Fig. 22 Insertion of the Drain connector, Luer-Lock-connectors and Blind plugs