Zeiss Lightsheet Z.1 Operating Manual

Contents

CHAPTER 1 - HARDWARELightsheet Z.1 User Interfaces Carl Zeiss02/2013 000000-1790-528 39Heating component Temperature range Incubation modulesHeating Block Heating from ambienttemperature to 42 °C(1) TempModule S1(2) Heating Block LSFMPeltier Block Heating and cooling in therange from 10 °C to 42 °C(1) TempModule S1(2) TempModule CZ-LSFM (including PeltierBlock and Temperature sensor)Tabelle 1 Overview Heating components3.7.2 CO 2-Module Lightsheet Z.1The CO2 -Module Lightsheet Z.1 controls and monitors adjustable CO2 concentration (from 0 % to 10 %)in the sample chamber and requires CO2 supply, sterile filter and Humidifier S1 to provide CO2 -enrichedair.− At least 3h (or more) may be needed until a stable pH value is reached due to the small surfacearea in relation to sample chamber volume.− For assembly of hardware components of the CO2 Module and its connections to the system, aswell as the calibration procedure needed follow the instructions from the PeCon manual.− After CO2 calibration, the Lightsheet Z.1 system must be completely turned off before work withthe system can continue.− Position the Luer-Lock connector for CO2 incubation at the top right corner of the sample chamber.Place a cover on top of the sample chamber body (Fig. 24, chapter 3.3.4) to maintain the CO2 gasconcentrations.− The sterility of the CO2-enriched air is given by the sterile filter. Take control of the sterility in yoursample chamber by following the cleaning instruction of all parts of the CO2 -module (see PeConmanual) as well as the components of the sample chamber (see CHAPTER 1 HARDWARE).Please clamp all incoming or outgoing tubing (Fig. 32/2, Fig. 32/5, Fig. 32/9, Fig. 32/10) into theprovided clamping grooves. This will avoid possible disconnection problems after closing the frontsystem cavity door.Note that for the sake of completeness Fig. 26 shows the connection diagram for the usage of aperfusion pump (Fig. 32/5, Fig. 32/9). Alternatively a syringe based-perfusion can be used (positionFig. 32/5). Consequently, Fig. 32/9 has to be replaced by a bling plug.