Zeiss Lightsheet Z.1 Operating Manual

Contents

CHAPTER 4 - SYSTEM OPERATIONLightsheet Z.1 Left Tool Area and Hardware Control Tools Carl Zeiss02/2013 000000-1790-528 41To have access to the respective controls, expandthe fields by pressing the triangles (Fig. 44).The Temperature ( oC) panel allows you to controlup to 2 independent heating channels that arelinked to certain devices (Peltierblock SampleChamber Lightsheet Z.1, Heatingblock SampleChamber Lightsheet Z.1) that are assigned todifferent channels. Enter the desired nominaltemperature via the input boxes by typing in thenumbers or by using the arrows to set theparameters. Check the corresponding box toactivate the desired Channel. The grey numberson the right will display the actual temperature.The Atmosphere (%) panel allows defining CO 2concentration via the input boxes. Either type inthe numbers or use the arrows to set theparameters. Actual values will be displayed on theright.3.2 Acquisition TabIf Acquisition is selected, the Acquisition panelbecomes available (Fig. 45).Acquisition contains all functions of the systemthat are needed to set up and control light sheetfluorescent imaging and multidimensional dataacquisition.Depending on the hardware configurationdetermines which of the described functions areavailable.For excitation, all imaging modes use lasers. TheAcquisition panel is divided into the− Experiment Manager field for loadingpredefined configurations, action buttonsand definition of the multidimensionality ofthe experiment and− Tool Group field with the tool tabs forsetting the imaging parameters.Fig. 44 Incubator panel – Expanded fields foratmosphere and temperature controlFig. 45 Acquisition panel – appearance