Zeiss Lightsheet Z.1 Operating Manual

Contents

CHAPTER 4 - SYSTEM OPERATIONLightsheet Z.1 Center Screen Area / Image Containers - Display and Image Analysis Carl Zeiss02/2013 000000-1790-528 2294.10.4 Overlap Coefficient after Manders(Manders, Verbeek and Aten, J. Microscopy 169:375-382, 1993)∑ ∑∑=i iiiiiiChChChChr 22 )2(*)1(2*1− Another parameter used to quantify colocalization in image pairs− Insensitive to differences in signal intensities between the two channels, photo-bleaching, oramplifier settingsValue range 0 – 1 (0: no colocalization, 1:all pixels colocalize)4.11 Profile ViewThis function allows to− display the intensity distribution of an imagealong a straight or curved line,− show the intensity values in table form andcopy table to clipboard or save as text file,− show separate profiles for each channel in amulti channel image.The settings of the Dimensions, Display, Playerand Graphics view options control blocks apply.The additional view-specific Profile View Optioncontrol block is shown in Fig. 341.Any changes done with this View Option control block are effective immediately.The content of the overlay plane is temporarily hidden while the toolbar is displayed.The Profile View can also be used online during imaging.• Draw a Profile line in the image display to define a profile. The intensity curves are shown in a separategraph (see Fig. 342).• In the Profile View Option control block you can select the width and type of the profile line. Thecolor setting is taken from the Graphics View Option control block.• You can place two markers on the profile line to measure differences in intensities and distances in theXY plane.• Tick the Profile in Image Display block to see an intensity graph superimposed on the image(see Fig. 343).Fig. 341 View Option control block - ProfileView